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Turkish Journal of Fisheries and Aquatic Sciences 2016, Vol 16, Num, 3     (Pages: 623-628)

Detection of Kudoa septempunctata in the Japanese Flounder by LoopMediated Isothermal Amplification (LAMP) Targeting the Internal Transcribed Spacer (ITS) Regions

Gouranga Biswas 1 ,Hirono Kodama 2 ,Tohru Mekata 3 ,Tomoya Kono 2 ,Hiroshi Yokoyama 4 ,Masahiro Sakai 2 ,Jun-ichi Hikima 2

1 Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan
2 Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-Nishi, Miyazaki 889-2192, Japan
3 Aquatic Animal Health Division, National Research Institute of Aquaculture, Fisheries Research Agency, Tsuiura, Saiki, Oita 879-2602, Japan
4 Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1- 1, Bunkyo-Ku, Tokyo 113-8657, Japan
DOI : 10.4194/1303-2712-v16_3_15 Viewed : 3630 - Downloaded : 3244 Recent food poisoning outbreaks from consumption of the Japanese flounder, Paralichthys olivaceus infested with a myxozoan parasite, Kudoa septempunctata have become a serious human health concern. In this study, we sequenced the internal transcribed spacer (ITS) regions between the 18S and 28S rRNA genes of K. septempunctata from cultured flounder in Japan and imported flounder from Korea. Sequence analysis indicates that the ITS1 regions are highly conserved in Japanese and Korean isolates of the parasite. A loop-mediated isothermal amplification (LAMP) assay targeting the ITS1 region was developed for rapid, sensitive and specific detection of either isolate of the parasite. Keywords : Kudoa septempunctata, ITS region, Japanese flounder (Paralichthys olivaceus), LAMP