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Turkish Journal of Fisheries and Aquatic Sciences 2003, Vol 3, Num, 2     (Pages: 131-138)

Molecular Diagnosis of Fish Diseases: a Review

İlhan Altinok 1 ,İlknur Kurt 1

1 Karadeniz Technical University, Sürmene Faculty of Marine Sciences, Department of Fisheries Technology Engineering, 61530 Surmene, Trabzon, Turkey Viewed : 4424 - Downloaded : 13655 Molecular techniques are potentially faster and more sensitive than culture, serology, and histology methods that are traditionally used to identify fish pathogens. During the last 15 years or so, molecular techniques have been increasingly employed to diagnose fish diseases. These techniques include polymerase chain reaction (PCR), restriction enzyme digestion, probe hybridization, in situ hybridization, and microarray. Pathogens can be detected from asymptomatic fish by molecular diagnostic techniques so disease outbreak could be prevented. Thus antibiotic treatment can be reduced so that creation of antibiotic resistant bacteria may be eliminated. In this paper molecular techniques for detection of fish pathogens are reviewed and the potential for their application are discussed. The application of new techniques as a routine tool in a diagnostic laboratory is an area where relevant literature is scarce and this may contribute to the reticence of some to adopt these methods. Keywords : Molecular techniques, fish diseases, antibiotic resistance, molecular diagnosis