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Turkish Journal of Fisheries and Aquatic Sciences 2022, Vol 22, Num, 1

Isolation and Toxigenic Characteristics of a Tetrodotoxin Producing Bacteria

Yong-jun Yuan 1 ,Feng Guan 1 ,Chen Yu 1 ,Zhuo Chen 1 ,Jie Zhang 1

1 Zhejiang Wanli University, College of Biological & Environmental Sciences, Ningbo/ Zhejiang, China DOI : 10.4194/TRJFAS19531 Viewed : 1656 - Downloaded : 1558 Tetrodotoxin (TTX) is a kind of neurotoxin with great scientific research and medicinal value. Studies have confirmed that microorganism is one of the important sources of TTX. However, the specific biosynthetic and regulatory pathway of TTX production by microorganisms are still unclear. In this paper, out of twelve symbiotic bacteria screened from the wild puffer fish (Fugu rubripes) collected from coastal sea area of Ningbo (Zhejiang, China), a strain named He-1 isolated from the liver of puffer fish was found can produce TTX. It was identified as Bacillus cereus based on physiological, biochemical characteristics and 16S rDNA gene analysis. However, the strain He-2, which is the subculture strain of He-1, cannot produce TTX under the normal culture condition. Further study showed that by adding the metabolite of the He-1, the ability of TTX producing of He-2 could be restored. A peptide compound was obtained by separating and purifying of the metabolites from strain He-1, which could induce the TTX production of strain He-2. This work will provide a new way for further revealing the anabolic pathway of TTX. Keywords : Tetrodotoxin Inducer Bacillus cereus Identification