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Turkish Journal of Fisheries and Aquatic Sciences 2021, Vol 21, Num, 2     (Pages: 95-105)

Establishment and Characterization of Eye Muscle Cell Line from Snow Trout, Schizothorax richardsonii (Gray, 1832), a Vulnerable Coldwater Fish, for In Vitro Studies

Murali Sanjeev Kumar 1 ,Pankaj Soni 1 ,Neha Singh 1 ,Ravindra Kumar 1 ,Shreya Srivastava1, 1 ,Akhilesh Kumar Mishra 1 ,Vijay Kumar Singh 1 ,Basdeo Kushwaha 1

1 Molecular Biology and Biotechnology Division ICAR-National Bureau of Fish Genetic Resources, Canal ring Road, P.O. Dilkusha, Lucknow-226002, Uttar Pradesh, India DOI : 10.4194/1303-2712-v21_2_05 Viewed : 419 - Downloaded : 396 Schizothorax richardsonii, commonly called snow trout is a vulnerable coldwater fish distributed in India and adjoining countries, is an important resource as food, ornamental and game fishery. A cell line, designated as SREM-1, has been established from the eye muscle of S. richardsonii using explant method and cultured in Leibovitz`s L-15 medium. The growth studies at 20-32°C temperatures and 5-20% of fetal bovine serum (FBS) concentrations revealed that SREM-1 cells exhibited optimal growth at 28°C in L-15 medium containing 20% FBS. Cell type was confirmed primarily as epithelial in nature by immuno-phenotyping assay and was sub-cultured for >55 times since its development. The origin of the cell line was confirmed by sequencing of cytochrome oxidase c subunit I and 16S rRNA genes. SREM-1 cell line is free from mycoplasma contamination and has been cryopreserved at different passage levels with revival efficiency of 70-80% after 6 months. The SREM-1 cell line was successfully transfected and also employed for evaluating in vitro cytotoxicity against a heavy metal, mercuric chloride. The newly established cell line of this vulnerable species would be useful as a model for aquatic toxicological and transfection studies. Keywords : Cell line, Cytotoxicity, India, Mercury chloride, NRFC, Schizothorax richardsonii