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Turkish Journal of Fisheries and Aquatic Sciences 2020, Vol 20, Num, 7     (Pages: 531-540)

Immune Responses of Three SOCS Genes in Yellow Catfish (Pelteobagrus fulvidraco) Challenged with Aeromonas hydrophila or Edwardsiella ictaluri

Hongyan Zhang 1-2 ,Xueying Pei 1-2 ,Yadong Hu 1-2 ,Zhaowen Wu 1-2 ,Xiang Zheng 1-2 ,Guosong Zhang 1-3 ,Tao Wang 1-2 ,Shaowu Yin 1-2

1 Nanjing Normal University, College of Marine Science and Engineering, College of Life Sciences Nanjing, Jiangsu 210023, China
2 Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu 222005, China
3 Heze University, School of agriculture and bioengineering, Key laboratory for physiology biochemistry and application, Heze, Shandong 274015, China
DOI : 10.4194/1303-2712-v20_7_03 Viewed : 2764 - Downloaded : 1718 Suppressors of cytokine signaling (SOCS) are a family of intracellular proteins emerging as key physiological regulators of cytokine responses in fish innate immune system. In this study, the tissue-specific distribution indicated that PfSOCS1, 2 and 3 were expressed ubiquitously and differentially in eight examined tissues. The highest transcript levels of PfSOCS1 and 3 respectively expressed in gill and liver, while that of the PfSOCS2 appeared mainly in muscle, followed by head kidney. The temporal patterns of PfSOCSs were assessed through the experimental challenge of A. hydrophila or E. ictaluri, and their expressions were altered in liver, gill and head kidney. Concretely, western blotting and quantitative real-time PCR analyses showed that PfSOCSs were significantly up-regulated in the early stage and then decreased after A. hydrophila or E. ictaluri challenge. Furthermore, the innate immune response of PfSOCSs in gill was more sensitive than that in liver and head kidney. PfSOCSs played vital roles in response to A. hydrophila or E. ictaluri challenge in three essential immune-related tissues. Our findings suggested that PfSOCSs played crucial roles in innate immunity of P. fulvidraco, and provided useful evidence for further understanding on the modulation mechanism of PfSOCSs in the innate immune system of P. fulvidraco Keywords : SOCS, Pelteobagrus fulvidraco, Aeromonas hydrophila, Edwardsiella ictaluri, Immune responses