Turkish Journal of Fisheries and Aquatic Sciences
2020, Vol 20, Num, 6 (Pages: 467-479)
Comparative Transcriptome Analysis of the Gills from the Chinese Mitten Crab (Eriocheir japonica sinensis) Exposed to the Heavy Metal Cadmium
Dan Tang 1 ,Huayun Guo 1 ,Xueling Shi 1 ,Zhengfei Wang 1
1 Yancheng Teachers University, School of Ocean and Biological Engineering, Yancheng, China
DOI :
10.4194/1303-2712-v20_6_05
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Heavy metal Cadmium (Cd) pollution has become a potential environmental problem,
and it threatens aquatic organisms including economic crabs and shrimps. Chinese
mitten crab is a native species in East Asia, and it is the most important economic crab
species in China. However, there are only a few studies about gills responding to Cd
stress in the Chinese mitten crab at a molecular level. To gain better understanding of
the oxidative stress system (antioxidant defense) of the E. j. sinensis, in this study, RNASeq was applied to obtain gene expression profile from the mitten crab`s gills in
response to Cd for 24h, and yielding 39,612,948 high-quality clean reads from which
570,847 transcripts and 504,889 unigenes are assembled by removing redundancy and
transcripts with low coverage. A total of 1544 differential expression genes (DGEs) are
identified. GO functional analysis and KEGG pathway analysis indicated some vital
pathways and genes in response to the challenge. Four genes related to immune
response and detoxification are selected to validate the DGE results by qRT-PCR. This
comparative transcriptome provides valuable molecular information about on the
mechanisms of the Chinese mitten crab responding to Cd that lays the foundation for
further understanding of heavy metals stress.
Keywords :
Transcriptome, Chinese mitten crab, Gill, Cadmium stress, Detoxification