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Turkish Journal of Fisheries and Aquatic Sciences 2019, Vol 19, Num, 7     (Pages: 585-592)

Innovative Approach of Asexual Reproduction Induction in Holothuria polii (Delle Chiaje, 1824)

Ragia Moussa Moussa 1 ,Lobna El-Saied Nasef 2 ,Hassan Abdel- Latif 2

1 National Institute of Oceanography and Fisheries, Aquaculture Division, Invertebrate Aquaculture Laboratory, Alexandria, Egypt
2 Alexandria University, Faculty of Science, Zoology Department, Egypt
DOI : 10.4194/1303-2712-v19_7_05 Viewed : 2790 - Downloaded : 2284 The current study describes a new method for asexual reproduction induction by fission. It results in higher rates of growth, survival and gut regeneration as compared to the conventional technique. Prior to asexual reproduction induction, samples were exposed to chemical evisceration induction through injection of 0.5 ml of 0.45M KCI into perivisceral coelomic cavity. Fission was induced in eviscerated group and normal (non-eviscerated) group through tightening the rubber bands around animal's body at the middle. We compared the modes of fission and the rates of growth, survival and gut regeneration over five months between two groups. We propose a new asexual reproduction induction method in which sea cucumber chemically induced to eviscerate prior to fission induction. Keywords : Fission Induction, Evisceration, Regeneration