Turkish Journal of Fisheries and Aquatic Sciences
2019, Vol 19, Num, 4 (Pages: 271-278)
Dietary Mannan Oligosaccharides Positively Affect the Growth, Digestive Enzyme Activity, Immunity and Resistance against Vibrio harveyi of Pacific White Shrimp (Litopenaeus vannamei) Larvae
Widanarni Widanarni 1 ,Abdurahman Taufik 1 ,Munti Yuhana 1 ,Julie Ekasari 1
1 Bogor Agricultural University, Department of Aquaculture, Faculty of Fisheries and Marine Science, Bogor 16680 Indonesia
DOI :
10.4194/1303-2712-v19_4_01
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This study aimed to determine the effect of dietary mannan oligosaccharides (MOS)
delivered through Artemia sp. at different concentrations (i.e. 0, 3, 6 and 12 mg L-1)
on the survival, growth, immunity, digestive enzymes and resistance against Vibrio
harveyi of Pacific white shrimp larvaein four treatment groups (in triplicates), i.e.
control, MOS3, MOS6 and MOS12. Larvae were reared in tank with a dimension of 25
cm × 20 cm × 30 cm containing 4 L of disinfected seawater at a stocking density of 30
larvae L-1. The larvae were maintained until they reached post larval stage for 15 days
and were subsequently challenged with V. harveyi (107 CFU mL-1) for 5 days. The
survival and growth of the shrimp in MOS treatments were significantly higher
(P<0.05) than control. Following challenge test with V. harveyi, the shrimp in MOS
treatments demonstrated higher survival, total haemocyte count (THC), phagocytic
activity, phenoloxidase and respiratory burst activities than those of the positive
control (P<0.05). The digestive enzyme activities in shrimp larvae fed with MOS were
higher than those in the control. The increase of digestive enzyme activity was
positively correlated with the increase of MOS concentrations with correlation
coefficients for protease, lipase and amylase were 0.96, 0.95 and 0.91, respectively
(P<0.05). In conclusion, the present study demonstrated that dietary MOS
supplementation through Artemia enrichment at the concentration up to 12 mg MOS
L-1 could significantly improve Pacific white shrimp post larval digestive enzymes
activities, growth, survival and resistance against V. harveyi infection.
Keywords :
Litopenaeus vannamei, Mannan oligosaccaharides, Prebiotics, Immune response, Vibrio harveyi