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Turkish Journal of Fisheries and Aquatic Sciences 2019, Vol 19, Num, 2     (Pages: 167-174)

The Timing and Characterization of Maternal to Zygote Transition and Mid-Blastula Transition in Sterlet Acipenser Ruthenus and A. ruthenus x Acipenser gueldenstaedtii Hybrid

Kseniia Pocherniaieva 1 ,Hilal Güralp 1 ,Taiju Saito 1 ,Martin Pšenička 1 ,Tomáš Tichopád 2 ,Karel Janko 2-3 ,Vojtěch Kašpar 1

1 University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic
2 University of Ostrava, Faculty of Science, Department of Biology and Ecology, Chittussiho 10, Ostrava, 710 00, Czech Republic
3 Institute of Animal Physiology and Genetics, Laboratory of Fish Genetics, CAS, v.v.i, Liběchov, 277 21, Czech Republic
DOI : 10.4194/1303-2712-v19_2_09 Viewed : 2995 - Downloaded : 3269 Detailed knowledge of early embryo development is a prerequisite for implementation of reproductive biotechnologies. We described the timing of mid-blastula transition in the sturgeon Acipenser ruthenus and hybrid of A. ruthenus and Acipenser gueldenstaedtii. To identify mitotic changes during cell division, the nuclei of embryo blastomeres were sampled every 30 min from 4 to 25 h post-fertilization and stained with 5 mg/ml 4`-6-diaminido-2- phenylindole. We observed embryos of hybrids to lose synchrony after the eighth cell division, one cycle earlier than in purebred sterlet embryos. To reveal the timing of zygotic gene activation, developing embryos were dechorionated and injected with 10, 50, 100, 250, or 500 µg/ml a-Amanitin. Termination of development in embryos was recorded throughout the period from the 1000 cell stage until gastrulation, showing the process of transition from maternal to zygotic gene transcription in sterlet to be of extended duration and not directly linked to mid-blastula transition. Keywords : Cell cycle, DAPI, Synchronization, Zygotic genome activation