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Turkish Journal of Fisheries and Aquatic Sciences 2018, Vol 18, Num, 9     (Pages: 1091-1100)

Cloning, Expression, and Functional Analyses of MHC Class II A in Ayu, Plecoglossus altivelis

Changhong Li 1 ,Feifei Ding 1 ,Jie Chen 1-2 ,Libin Mo 1 ,Jiong Chen 1-2

1 Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo, China
2 Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, China
DOI : 10.4194/1303-2712-v18_9_08 Viewed : 3889 - Downloaded : 2417 The MHC (major histocompatibility complex) class II family plays a major role in vertebrate immune systems and is normally found only on antigen-presenting cells. In this study, we identified the gene encoding the α (PaMHCIIα) chain of MHC class II molecules in ayu (Plecoglossus altivelis). Phylogenetic analysis showed that PaMHCIIα was tightly grouped with MHC class IIα in rainbow smelt (Osmerus mordax) and salmon. PaMHCIIα transcripts were detected in all tested tissues, with high levels in the spleen, head kidney, and gill; expression increased significantly upon Vibrio anguillarum infection. PaMHCIIα mRNA expression was also up-regulated in monocytes/macrophages (MO/MФ) after in vitro stimulation with V. anguillarum. We prepared antibodies for detecting N-glycosylation of PaMHCIIα or neutralizing PaMHCIIα on ayu MO/MФ. Western blot analysis showed that native PaMHCIIα was indeed N-glycosylated. Moreover, PaMHCIIα neutralization not only significantly inhibited ayu MO/MФ phagocytosis, but also reduced the ability of V. anguillarum to induce MO/MФ-cytokine expression. Overall, these findings reveal that the important role of PaMHCIIα against bacterial challenge, and it likely contributes to pathogen responsiveness in ayu MO/MФ.. Keywords : Ayu, MHC class II α,vibrio anguillarum,gene expression, monocyte/macrophage functions