Turkish Journal of Fisheries and Aquatic Sciences
2018, Vol 18, Num, 2 (Pages: 229–237)
Short-Term Effects of Human Kisspeptin on LH Secretion in Prussian Carp (Carassius gibelio Bloch, 1782) Females at Two Gonad Maturity Stages
Mirosława Sokołowska-Mikołajczyk 1 ,Grzegorz Gosiewski 1 ,Jaroslaw Chyb 1 ,Magdalena Socha
1 University of Agriculture, Department of Ichthyobiology and Fisheries, ul. Prof. T. Spiczakowa 6, 30-198 KrakowMydlniki, Poland
DOI :
10.4194/1303-2712-v18_2_02
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Kisspeptin plays a critical role in the control of reproduction and puberty onset in vertebrates, fish including. To
demonstrate the pattern of LH secretory responses [spontaneous and stimulated by GnRH analogue (Des Gly10, D-Ala6
ethylamide LHRH)] to single, intraperitoneal injection of human kisspeptin [metastin (45-54) amide], blood samples from
mature Prussian carp females (at the time of gonad recrudescence and natural spawning) were collected at 5, 10, 15 and 30
minutes after injection. To test the influence of dopamine on kisspeptin action on LH release, the dopamine antagonist -
pimozide - was used together with kisspeptin and GnRH analogue. At the time of gonad recrudescence kisspeptin alone had
no effect on LH secretion. At the time of natural spawning the transient increase of LH levels at 10th minute post-injection was
observed. Also the combination of kisspeptin and GnRHa increased LH levels in comparison to control fish at 10, 15 and 30
minutes after injections and at 10th minute kisspeptin potentiated stimulatory effects of GnRHa. No influence of kisspeptin on
the spontaneous, as well as GnRHa-stimulated LH release was observed in pimozide-treated fish. Presented data suggest that
the short-term response to kisspeptin is dependent on the stage of gonadal maturity
Keywords :
Prussian carp, human kisspeptin, LH levels