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Turkish Journal of Fisheries and Aquatic Sciences 2016, Vol 16, Num, 3     (Pages: 637-642)

Characterization of Proteins from Popovo minnow (Delminichthys ghetaldii Steindachner, 1882) Muscle

Radoslav Dekić 1 ,Jasna Friščić 1 ,Aleksandar Ivanc 2 ,Biljana Kukavica 1

1 University of Banja Luka , Faculty of Science and Mathematics, Mladena Stojanovica 2, Banja Luka, Bosnia and Herzegovina
2 State University of Novi Pazar, Department of Biomedical Sciences, Vuka Karadzica bb, Novi Pazar, Serbia
DOI : 10.4194/1303-2712-v16_3_17 Viewed : 1945 - Downloaded : 1836 In this research protein profiles of Popovo minnow muscle were presented and partially characterized for the first time. Delminichthys ghetaldii (Steindachner, 1882) is an endemic fish species which can be found in springs and streams in fields Ljubomir, Dabar and Fatnica in Bosnia and Herzegovina, and Bacina lakes in Croatia. Most of the year minnows dwell in underground water habitats of karst region in eastern Herzegovina. During the autumn and spring flooding of the surrounding fields, Popovo minnows emerge from the underground waters in estavele and spawn in newly formed temporary lakes. In order to obtain protein profiles from fish muscle, we used several extraction protocols and buffers. Sarcoplasmic proteins were extracted from muscle tissue using Tris/EDTA and Tris/CaCl2 buffers. The results showed that the most abundant sarcoplasmatic proteins had molecular weight of 40 and 34 kDa while high MW band appeared only in samples extracted by Tris/EDTA. Re-extraction with 1% SDS resolved the presence of myofibrillar proteins and showed to be qualitatively and quantitatively more efficient than the extraction by nonionic detergent Tween. Our results also indicate that protein extraction with commercial SDS buffer from a biopsy muscle tissue gives good screening results and is applicable for field work. The results are discussed in terms of the efficiency of different buffers in protein extraction from Popovo minnow muscle tissue. Keywords : Delminichthys ghetaldii, muscle, Popovo minnow, protein profile