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Turkish Journal of Fisheries and Aquatic Sciences 2016, Vol 16, Num, 2     (Pages: 461-467)

MRF Gene Family in Schizothorax prenanti: Molecular Cloning, Tissue Expression, and mRNA Expression in Muscle Development

Yaqiu Lin 1 ,Jishu Zhou 2 ,Ruiwen Li 3 ,Xianrong Xiong 2 ,Pengfei Wu 1

1 Southwest University for Nationalities, College of Life Science and Technology, Chengdu, 610041, P.R. China
2 Northwest A & F University, College of Animal Science and Technology, Yangling 712100, P.R. China
3 Chengdu Woman-Child Central Hospital, Reproductive and endocrine laboratory, Chengdu 610091,P.R. China
DOI : 10.4194/1303-2712-v16_2_26 Viewed : 2321 - Downloaded : 1434 The myogenic regulatory factors (MRFs) play key roles in growth and muscle development in mammals and some fish, however previous studies about the cloning, tissue expression and mRNA expression in muscle development of MRFs genes in Schizothorax prenanti (S.prenanti) was scarce. In order to know how MRFs genes were expressed in different tissues and development period, we cloned the complete CDS of the MRF gene family from S.prenanti for the first time, including MyoD, MyoG, Myf5 and Myf6. Amino acid sequence alignment indicated that the S.prenanti MRF gene family has a basic helixloop- helix (HLH) domain. Quantitative real-time RT-PCR analysis indicated that MyoD, MyoG, Myf5 and Myf6 mRNA was highly expressed in red muscle and white muscle. The mRNA expression of MRF family in muscle during different developmental stages (larvae, juvenile and adult) in vivo were also investigated. The results showed that MyoG and Myf5 expression in muscle were significantly associated with muscle development. Our findings provided molecular characterization and expression profile of the MRF gene family in S.prenanti and enhanced understanding about the MRFs in muscle growth and development in fish. Keywords : MRFs gene family; muscle development; gene expression; Schizothorax prenanti