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¹ Bharathiar University, Department of Zoology, Crustacean Biology Laboratory, Coimbatore-641046, Tamilnadu, India DOI : 10.4194/1303-2712-v12_2_19 Viewed : 4188 - Downloaded : 4479 The present study was attempted to examine the combined effects of probiotics, Lactobacillus sporogenes (LS), Bacillus subtilis (BS) and yeast, Saccharomyces cerevisiae (SC) on survival, growth, biochemical changes and energy utilization performance of the freshwater prawn M. rosenbergii post larvae. The probiotics, L. sporogenes (4), B. subtilis (3) and S. cerevisiae (4) were taken and mixed. 1%, 2%, 3% and 4% of LS+BS+SC (4+3+4) was incorporated with basal diet. Diet without probiotics served as control. PL-30 of M. rosenbergii was fed with LS+BS+SC (4+3+4) incorporated diet for a period of 90 days. The growth parameters, such as survival, weight gain, specific growth rate, feed conversion efficiency and protein efficiency rate were significantly (P<0.05) higher in 3% LS+BS+SC incorporated diet fed post larvae (PL) followed by other experimental groups when compared with control. Whereas, the feed conversion ratio was significantly (P<0.05) lower in 3% LS+BS+SC incorporated diet fed PL. Similarly the tissues biochemical composition such as protein, amino acid, carbohydrate and lipid content were significantly (P<0.05) higher in 3% LS+BS+SC incorporated diet fed PL. However, insignificant difference was recorded in moisture content between control and experimental groups. The energy utilization parameters, such as feeding rate, absorption rate, conversion rate, NH₃ excretory rate and metabolic rate were significantly (P<0.05) higher in 3% LS+BS+SC incorporated diet fed PL. Therefore, the probiotics 3% LS+BS+SC incorporated diet fed PL was produced better growth performance. Keywords : M. rosenbergii, L. sporogenes, B. subtilis, S. cerevisiae, growth performance, biochemical constituents, energy utilization