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Turkish Journal of Fisheries and Aquatic Sciences 2020, Vol 20, Num, 7     (Pages: 541-548)

Genetic Variation of Insulin-like Growth Factor II (IGF-II) Gene and its Associations with Growth Traits in European Sea bass (Dicentrarchus labrax)

Emel Ozcan-Gokcek 1 ,Raziye Isik 2 ,Bilge Karahan 1 ,Kutsal Gamsiz 1

1 Ege University, Faculty of Fisheries, Department of Aquaculture, İzmir, Turkey
2 Tekirdağ Namık Kemal University, Faculty of Agriculture, Department of Agricultural Biotechnology, Tekirdağ, Turkey
DOI : 10.4194/1303-2712-v20_7_04 Viewed : 1003 - Downloaded : 746 Insulin-like growth factor II (IGF-II) is a peptide hormone which plays a crucial role in the growth and metabolic regulations of vertebrates. In this study, we identified single nucleotide polymorphisms on IGF-II gene in the European sea bass (Dicentrarchus labrax) and revealed the potential association between the genotypes of IGF-II gene and growth traits in 150 European sea bass. The polymorphisms were determined using DNA sequencing and PCR-RFLP methods in two different regions of IGF-II gene. An AT indel and two SNPs as g.5127711C>G and g.5127731G>T in the intron 2 region, and one SNP as g.5129188C>T in the intron 3 region of IGF-II gene in the European sea bass were reported for the first time with this study. Two different genotypes (GG, TG) were observed and the G allele was predominant (65%) in the IGF-II-NdeI locus. Associations between the genotypes of IGF-II-NdeI locus and body weight and total length were statistically significant (P<0.05). Body weight and total length in each group were higher for GG genotype of IGF-II-NdeI locus (p<0.05). In conclusion, the IGF-II-NdeI locus had a significant effect on the body weight and total length which could be useful for sea bass selection and breeding for marker-assisted selection program. Keywords : IGF-II Dicentrarchus labrax SNP Gene Growth Traits