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Turkish Journal of Fisheries and Aquatic Sciences 2019, Vol 19, Num, 4     (Pages: 343-349)

Development of Polymorphic Microsatellite Markers from AFLP Products in the Xinjiang Arctic Grayling (Thymallus arcticus grubei) and a Test of Cross-Species Amplification

Yun-Guo Liu 1-2 ,Ling-Xiao Liu 2 ,Wen-Juan Shan 1 ,Jun Li 1 ,Wei Meng 1 ,Yao-Yao Li 1 ,QingDian Han 2 ,Xiao-Ming Qu 3

1 Xinjiang University, College of Life Sciences and Technology, Urumqi 830046, China
2 Linyi University, College of Life Sciences, Linyi 276000, China
3 Liaoning Center for Disease Control and Prevention, Shenyang 110005, China
DOI : 10.4194/1303-2712-v19_4_08 Viewed : 598 - Downloaded : 197 The population of Xinjiang arctic graylings (Thymallus arcticus grubei) has dramatically declined in China. A total of 22 polymorphic microsatellite loci were isolated and characterized from AFLP products in the Xinjiang arctic grayling. The number of alleles (Na) per locus varied from 3 to 13. Observed (HO) and expected (HE) heterozygosities ranged from 0.60 to 0.97, and from 0.58 to 0.89, respectively. These new microsatellite markers are a helpful tool for genetic analyses, stock management, and resource conservation efforts for T. arcticus grubei. According to the total number of alleles, the total effective number of alleles, and the total number of genotypes, the Fuhai population showed the highest diversity among all the populations while the Fuyun sample was the lowest. Five additional fish species: Red seam bream (Pagrus major), Lenok (Brachymystax lenok), Sea perch (Lateolabrax japonicus), Taimen (Hucho taimen), and Amur grayling (Thymallus grubii) were assessed for cross-species amplification. Three out of the five species showed at least two polymorphic loci. In addition, six loci were found to be polymorphic in at least one species. Keywords : Thymallus arcticus grubei, Microsatellite markers, Cross-species amplification