Turkish Journal of Fisheries and Aquatic Sciences
2017, Vol 17, Num, 1 (Pages: 111-120)
Effect of the Activation Media with Different Osmolality and Cool Storage on Spermatozoa Motility Parameters Over Time in Zebrafish, Danio rerio
Sara Sadeghi 1 ,Javier Nuñez 1 ,Carles Soler 1 ,Miguel Angel Silvestre 1
1 Departamento de Biología Funcional y Antropología Física, Universitat de Valencia, Burjassot, Valencia, Spain
DOI :
10.4194/1303-2712-v17_1_13
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In the present work, the effects of the activation media with different osmolality and storage conditions on sperm motility and
kinetic parameters over time after activation, using CASA system (ISAS®) in Danio rerio were analysed. The effect of pooled
and individual ejaculate samples was also studied. Spermatozoids activated with high osmolality medium showed higher
values in motility parameters and kinematic parameters in comparison with low osmolality medium. Respect to the individual
ejaculates or the pool, although significant differences were observed in some parameters, no relevant changes were observed
in total motility neither in kinetic parameter. Total motility and velocity of sperm showed significant lower values after sperm
storage for 24 h at 4 ºC.
Keywords :
Sperm motility, activation, osmolality, zebrafish, CASA, kinetic parameters