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Turkish Journal of Fisheries and Aquatic Sciences 2016, Vol 16, Num, 4     (Pages: 789-795)

Molecular Identification of Astacus leptodactylus and Austropotamobius torrentium Using mtDNA-RFLP Method

Gökhan Kalaycı 1 ,Süleyman Akhan 2

1 Recep Tayyip Erdoğan University, Faculty of Fisheries, Rize, Turkey
2 Akdeniz University, Faculty of Fisheries, Antalya, Turkey
DOI : 10.4194/1303-2712-v16_4_05 Viewed : 3994 - Downloaded : 3789 mtDNA-RFLP, a technique commonly used for molecular identification of species, was used to distinguish between morphologically similar crayfish species Austropotamobius torrentium and Astacus leptodactylus in this study. The mitochondrial COI gene was amplified and digested using 15 restriction endonucleases to establish a restriction map. The COI gene sequence contained recognition sites for three of the restriction endonucleases (SspI, KpnI, and RsaI) and produced two fragments and three genotypes for Austropotamobius torrentium. However, the Astacus leptodactylus COI gene did not have restriction sites for these three enzymes. Thus, a mtDNA-RFLP using these three endonucleases can be used to successfully distinguish between these two species of crayfish. Keywords : crayfish, species identification, mtDNA, COI