Turkish Journal of Fisheries and Aquatic Sciences
2014, Vol 14, Num, 2 (Pages: 515-525)
Comparative Study of the Effects of Two High-Carbohydrate Diets on Growth and Hepatic Carbohydrate Metabolic Enzyme Responses in juvenile GIFT tilapia (Oreochromis niloticus)
J. Qiang 1 ,H. Yang 1 ,J. He 1 ,H. Wang 1 ,Z.X. Zhu 1 ,P. Xu 1
1 Ministry of Agriculture, Freshwater Fisheries Research Center, Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Chinese Academy of Fishery Sciences, Wuxi 214081, Jiangsu, China
DOI :
10.4194/1303-2712-v14_2_23
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This study aimed to compare the effect of two high-carbohydrates diets on growth, activity and gene expression of
hepatic metabolic enzymes in GIFT tilapia juveniles (Oreochromis niloticus). Two isonitrogenous (28% crude protein) and
isolipidic (5% crude lipids) diets were formulated to contain 40% of wheat starch (WS) or glucose (GLU). Diets were
assigned to triplicate groups of 30 fish (initial weight: 11.26g) for 45 days. At the end of trial, SGR, feed intake, feed
efficiency, HSI, VI and liver glycogen levels were not significantly influenced by dietary carbohydrates. Plasma glucose was
higher in fish fed the GLU diet than in the WS diet. No significant effect of dietary carbohydrates source on GK and G6Pase
activities were present, however, these activities were significant higher than the initial levels. In contrast, higher G6PD
activities were recorded in GLU group than in the WS group on day 45. Data on gene expression showed that G6Pase and
G6PD but not GK was affected by dietary carbohydrates on day 45; the levels of G6Pase and G6PD mRNA in GLU group
were significantly higher than in WS group. Overall, dietary high glucose levels was utilized well as energy source by GIFT
tilapia juveniles, and seem to be more effective in increasing liver lipogenic activities.
Keywords :
Oreochromis niloticus; carbohydrate metabolism; enzyme activity; gene expression; growth