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Turkish Journal of Fisheries and Aquatic Sciences 2012, Vol 12, Num, 1     (Pages: 185-187)

Elimination of Pathogenic Bacterium (Micrococcus sp.) by the Use of Probiotics

Tejpal Dahiya 1 ,S. K. Gahlawat 2 ,R. C. Sihag 3

1 Department of Zoology, Kurukshetra University, Kurukshetra-136119 (Haryana) India
2 Department of Biotechnology, Chaudhary Devi Lal University, Sirsa-125055 (Haryana) India
3 Department of Zoology and Aquaculture, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004 (Haryana) India
DOI : 10.4194/1303-2712-v12_1_21 Viewed : 3814 - Downloaded : 3553 Probiotics 1 (Lactobacillus sporogenes), 2 (Saccharomyces boulardii) and 3 (Nitromonas, Rhodococcus, Bacilus megaterium, Lecheni formis, Desulphovibrio sulphuricum, Psuedomonas, Chromatium, Chlorobium, Thiobacillus, Thioxidants, Thiobacilus ferroxidant, Methylomonas methyanica, Glucon acetobactor, Azospirillum, Trichoderma, Shizophyllum commune and Sclertium gluconicum) were tested against the pathogenic Micrococcus sp.oxt in vitro as well as in vivo for four weeks. In vitro experiment revealed that the zone of inhibition of probiotic 1 was higher than probiotic 3 followed by probiotics 2. In vivo experiment also revealed that the elimination of pathogenic Micrococcus sp.oxt from 1.54 x 1011 colony forming units (cfu)/ml to 2.50 x 101 by probiotic 1 was higher than; to 2.00 x 101 cfu/ml by probiotic 3 and; to 3.3 x 101 cfu/ml by probiotics 2. The present investigation confirmed the elimination of pathogenic bacterium, Micrococcus sp.oxt in both in vitro and in vivo experiments. Keywords : Probiotics, Lactobacillus sporogenes, CFU (Colony Forming Units), Micrococcus sp., zone of inhibition