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Turkish Journal of Fisheries and Aquatic Sciences 2013, Vol 13, Num, 1     (Pages: 59-168)

Comparative Analysis of Using Isozyme and Issr-Pcr Markers for Population Differentiation of Cyprinid Fish

Oxana Nikolaevna Zhigileva 1 ,Oxana Georgievna Baranova 1 ,Viktor Valentinovich Pozhidaev 1 ,Ivan Sergeevich Brol 1 ,Tatyana Ivanovna Moiseenko 2

1 Tyumen State University, Department of Ecology and Genetics, Pirogova St., 3, Tyumen, 625003 Russian Federation
2 V.I. Vernadsky Institute of Geochemistry and Analytical Chemistry of RAS Kosygin Street 19, Moscow, Russian Federation
DOI : 10.4194/1303-2712-v13_1_19 Viewed : 4401 - Downloaded : 3593 The article presents data on genetic variability and differentiation of three species of cyprinid fish – roach Rutilus rutilus, ide Leuciscus idus and dace Leuciscus leuciscus baikalensis in the rivers of Siberia, Russia. From the three species studied ide has the lowest indices of genetic variability and more pronounced population differentiation. About 40% of isozyme loci are polymorphic in this species and the same percent – for nuclear DNA inter-simple sequence repeat (ISSR) markers; interpopulation component accounts for 62% of genetic variability. In roach indicators of genetic variability are higher, especially for DNA markers (67%), and interpopulation differentiation is less pronounced (FST = 0.550; GST = 0.214). Dace has the most significant genetic diversity and low differentiation of populations. 48% of the isozyme loci and 83% of multilocus DNA markers are polymorphic in this species, the interpopulation component accounts for 27% and 19% for two types of markers, respectively. In comparison with allozymes, ISSR markers provide higher estimates of genetic distances while making intrageneric comparisons and similar or somewhat lower values – while making a comparison of different groups. Isozyme markers provide more precise geographical differences of population groups of fish. Keywords : Cyprinid fish, Siberia rivers, Genetic differentiation, Allozymes, ISSR