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Turkish Journal of Fisheries and Aquatic Sciences 2026, Vol 26, Num, 5     (Pages: TRJFAS28407)
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Selection of Probiotic Isolated from Marine Species

Safa Jedidi 1-2-3 ,Amal Dbeibia 1 ,Ahmed Ben Nacib 1-2 ,Tarek Zmanter 1 ,Lamia Ayed 1-2 ,Mohamed Mansour Marzouki 3 ,Kamel Hadj Mbarek 3 ,Ridha Mzoughi 1 ,Chedia Jabeur 1-2

1 Laboratory of analysis, treatment and valorization of pollutants of environment and products. Faculty of pharmacy of Monastir- Rue Avicenne, 5000 Monastir, Tunisia
2 High Institute of biotechnology of Monastir, 5000 Monastir, Tunisia
3 Technical Center of Aquaculture, 1009 Montfleury, Tunis, Tunisia DOI : 10.4194/TRJFAS28407 Viewed : 72 - Downloaded : 90 The objective of this study is to investigate the probiotic potential of marine lactic acid bacteria (LAB) by isolating them from a range of fish samples, identifying the different strains, and analysing their probiotic characteristics. Using 16S rDNA sequencing, three LAB strains were identified and evaluated for their probiotic potential, including their enzymatic activities (such as hemolytic and DNase activities, and API ZYM profile) and their resilience to gastrointestinal conditions. The study also examined their antimicrobial properties, adhesion abilities (including biofilm formation capacity), auto-aggregation, co-aggregation with pathogenic bacteria, and surface hydrophobicity. Their antibiotic susceptibility was also evaluated.

The LAB strains; Saf1 (Lacticaseibacillus paracasei), Saf2 (Pediococcus pentosaceus), and Saf3 (Pediococcus acidilactici) demonstrated significant probiotic potential, tolerating harsh gastric and intestinal conditions (low pH, pepsin, and trypsin resistance) without exhibiting detectable DNase or hemolytic activity. Furthermore, all strains displayed strong auto and co aggregation capacities, and high hydrophobicity. The tested isolates effectively inhibited various fish pathogens isolated from seabass and seabream, including Vibrio alginolyticus, Vibrio vulnificus, Vibrio parahaemolyticus, Aeromonas salmonicida and Aeromonas hydrophila. In addition, all isolates were sensitive to veterinary antibiotics and produced phosphatase enzymes, which support growth, boost immunity, and ensure proper mineral absorption, making probiotics an attractive alternative to traditional antibiotics in aquaculture systems. Overall, the findings of this current investigation indicate that Lacticaseibacillus paracasei (Saf1) is the most promising probiotic strain, which could be exploited for functional use in aquaculture. Keywords : Probiotic Aquaculture Fish pathogens, Lactic Acid Bacteria