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¹ Industrial University of Ho Chi Minh City, Institute of Biotechnology and Food Technology, Ho Chi Minh city, Vietnam (700000) DOI : 10.4194/TRJFAS28287 Viewed : 8 - Downloaded : 9 To evaluate the dual antioxidant and antiparasitic effects of Melia azedarach leaf ethanol extract (EMAE) in whitespotted catfish (Clarias fuscus). In vitro, six groups assessed DPPH/FRAP and Trichodina nigra growth inhibition; in vivo, infected fish received formalin (100 mg/L, 30 min/day ×3) or EMAE baths (800-1000 ppm, 60 min/day ×3). EMAE showed dose-dependent antioxidant activity (EMAE1000: DPPH 49.43%; FRAP 0.56 mmol Fe²⁺/g). In vitro, EMAE1000 inhibited parasite growth (20.98% at 72 h). In vivo, EMAE reduced T. nigra intensity (skin 18.67; gills 27.67 at 1000 ppm) and improved growth (BWG 37.86%; SGR 1.24% day⁻¹). Oxidative stress markers (NO, O₂⁻) decreased, while antioxidant enzymes (PON, TrxR, POD) increased toward control levels. EMAE provides measurable antiparasitic and redox-modulating benefits in C. fuscus, supporting its potential as a sustainable adjunct to conventional treatments. Keywords : Aquaculture, Clarias fuscus, Melia azedarach, Oxidative stress, Trichodina nigra