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Turkish Journal of Fisheries and Aquatic Sciences 2022, Vol 22, Num, 2     (Pages: TRJFAS19802)

Effect of Leucas aspera Against Aeromonas hydrophila in Nile Tilapia (Oreochromis niloticus): Immunity and Gene Expression Evaluation

Preetham Elumalai 1 ,Amitha Kurian 1 ,Sreeja Lakshmi 1 ,Mohamed Saiyad Musthafa 2 ,Einar Ringo 3 ,Caterina Faggio 4

1 Kerala University of Fisheries and Ocean Studies, School of Ocean Science and Technology, Kochi/Kerala, India
2 The New College, Chennai, Department of Zoology, Chennai, India
3 The Arctic University, Faculty of Biosciences, Tromso, Norway
4 University of Messina, Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Messina, Italy
DOI : 10.4194/TRJFAS19802 Viewed : 119 - Downloaded : 114 The present study addressed the effects of Leucas aspera enriched diet in Nile tilapia. Three hundred Nile tilapia were fed Leucas aspera as follows: 0 g kg-1 L. aspera (C-control), 5 g kg-1 L. aspera (T1), 10 g kg-1 L. aspera (T2) and 15 g kg-1 L. aspera (T3). After 30 days of feeding, significant (P<0.05) increase in growth performance was noticed by feeding the fish the T2 diet. Thereafter fish were intraperitoneal injected with Aeromonas hydrophila in challenge test. After 21 days of challenge, highest survival rate (70%) was observed in fish fed the T3 diet followed by fish fed T2 diet (65%). Serum immunological parameters such as phagocytosis, alternative complement activity, respiratory burst activity and lysozyme activity were significantly (P<0.05) enhanced in fish fed all inclusion levels of L. aspera with the maximum activity in fish fed the T2 diet. Hematological parameters were significantly (P<0.05) higher in all groups fed L. aspera diets vs. control fed fish. No histopathological changes in liver were observed in fish fed the T2 diet in the histology study. Gene expression study revealed the upregulation in the expression of COX-2 and GR genes. In conclusion, the current results suggest that dietary administration of L. aspera especially the T2 diet, has beneficial effects in improving immunity and can mitigate the adverse effects of A. hydrophila infection in Nile tilapia. Keywords : Leucas aspera Nile tilapia Growth performance Serum immunology Disease resistance Hematology