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Turkish Journal of Fisheries and Aquatic Sciences 2019, Vol 19, Num, 12     (Pages: 1049-1059)

Sequencing and Transcriptome Analysis for Reproduction-Related Genes Identification and SSRs Discovery in Sequential Hermaphrodite Amphiprion ocellaris

Wei Yang 1-4 ,Baian Lin 3 ,Guangli Li 1 ,Huapu Chen 1-2 ,Min Liu 3

1 Zhanjiang City Key Laboratory for Marine Ecology and Environment, Guangdong Research Center for Reproductive Control and Breeding of Indigenous Valuable Fish Species, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China
2 Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
3 State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China
4 Food and Environmental Engineering Department, Yangjiang Polytechnic, Yangjiang 529566, China
DOI : 10.4194/1303-2712-v19_12_07 Viewed : 3032 - Downloaded : 2827 Amphiprion ocellaris is suggested to be an excellent research model for understanding socially controlled sex change of sequential hermaphrodite teleosts. However, there is a lack of knowledge regarding the genes involved in reproduction regulation in sex transition process. In order to enrich genetic data and sequence resources, Illumina HiSeq transcriptome sequencing was conducted for fully mature A. ocellaris using a multiple tissues mixing strategy. A total of 109,408,556 raw reads were obtained and 81,395 unigenes were generated by Trinity de novo assembly. Of which, 60,457 unigenes were annotated in at least one of the queried NR, COG, Swiss-Prot, KEGG and NT databases. After screening from reproduction-related Gene Ontology (GO) terms and pathways, plentiful candidate genes involved in reproductive process were identified, such as gnrh1, fsh, mtnr1a, avt, dmrt1, sox9a, amh, dax1, sf-1, foxl2 and cyp19a1. These candidates could make good starting points for profound mechanical analysis on the reproductive regulation during sex change. Moreover, 18,037 potential simple sequence repeats (SSRs) were detected in 14,212 unigenes for marker development. This transcript dataset will greatly increase available resources for gene mining and function analysis, and could contribute valuable information to in-depth studies on reproductive biology, genetics and genomics in the future. Keywords : False clown anemonefish, Amphiprion ocellaris, Sex change, Reproductive regulation, Candidate gene