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Turkish Journal of Fisheries and Aquatic Sciences 2019, Vol 19, Num, 2     (Pages: 141-147)

Antibiotic Resistance and Virulence-Associated Gene Profiles of Edwardsiella tarda Isolated from Cultured Fish in Japan

Sudu Hakuruge Madusha Pramud Wimalasena 1 ,Hansani Nilupama Kumari Senarath Pathirana 1 ,Benthotage Chamara Jayasankha De Silva 1 ,Sabrina Hossain 1 ,Emi Sugaya 2 ,Toshihiro Nakai 3 ,Gang-Joon Heo 1

1 Chungbuk National University, Veterinary Medical Center and College of Veterinary Medicine, Cheongju, 28644, Korea
2 Fukuyama University, Faculty of Life Science and Biotechnology, Fukuyama, Hiroshima 729-0292, Japan
3 Hiroshima University, Graduate School of Biosphere Science, Higashi-Hiroshima, Hiroshima 739-8528, Japan
DOI : 10.4194/1303-2712-v19_2_06 Viewed : 6902 - Downloaded : 5009 Edwardsiella tarda is one of the most significant bacterial pathogens to fish aquaculture worldwide. Here, we evaluated antibiotic resistance profiles, virulence genotypes and phylogenetic relationship of 30 Edwardsiella tarda isolates derived from cultured fish in Japan. The E. tarda isolates were phylogenetically (16S rRNA) clustered into two clads. The isolates of clad 1, which were mainly originated from olive flounder Paralichthys olivaceus and Japanese eel Anguilla japonica, had all of the known 11 virulence genes (ank, katB, LuxS, citC, astA, evpP, mukF, gadB, fimA, hlyA, esa) for E. tarda and mostly one or two of three aminoglycoside resistance genes, i.e. aac(6')-Ib, armA and strA-strB, if exists. In contrast, the isolates of clad 2, which were mainly originated from red seabream Pagrus major and black seabream Acanthopagrus schlegelii, lacked four (ank, katB, LuxS, citC) of the 11 virulence genes and, eight of them harbored mainly a β-lactam resistance gene (blaCTX-M). In infection experiments with zebrafish, representative isolates of the clad 1 showed higher virulence than those of the clad 2. These results reveal that the virulence profiles may be applicable for prediction of pathogenicity in fish. Phylogenetic analysis revealed that E. tarda isolates derived from olive flounder and red seabream are ancestor differentiated. Keywords : Edwardsiella tarda, Edwardsiellosis, Antibiotic resistance genes, Virulence gene, Pathogenicity, Phylogenetics, Paralichthys olivaceus, Pagrus major