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Turkish Journal of Fisheries and Aquatic Sciences 2017, Vol 17, Num, 7     (Pages: 1367-1372)

The Effects of Salinity and Temperature on the Growth of Dunaliella sp. Isolated from the Salt Lake (Tuz Gölü), Turkey

Imen Hamed 1 ,Burcu Ak 2 ,Oya Isık 2 ,Leyla Uslu 2

1 Biotechnology Research and Application Centre, Cukurova University, Adana, Turkey
2 Department of Basic Sciences, Faculty of Fisheries, Cukurova University, Adana, Turkey
DOI : 10.4194/1303-2712-v17_6_29 Viewed : 3226 - Downloaded : 3174 In this study Dunaliella sp. was isolated from the hypersaline Lake Tuz and its cell density, dry matter, chlorophyll a, and β-carotene production were studied in a batch system. The aim of this research was to determine the optimal conditions for Dunaliella sp. growth. Therefore 3 different temperatures (18°C, 22°C, and 26°C) with 3 different salinities (25‰, 30‰, and 35‰) were tested over a 19-day study. Significant differences were found at the end of the cultivation period (P<0.05) for 18°C and 26°C. No statistically significance were reported for all tested salinity degrees (P>0.05). At the end of the experiment the maximum growth were noticed for 18°C 25‰ in which the culture reached 3.48 105 cell/ml. The lowest was reported for 26°C 35‰ (1.50 105 cell/ml). The culture of 18°C 25‰ had also the best chlorophyll a concentration and biomass amount. For the β-Carotene 22°C 30‰ (4.39 10 -6 mol/l) had the highest accumulation. The optimum temperature for Dunaliella sp. growth was 18°C at 25‰ Keywords : Dunaliella sp, stress factors, pigment analysis, density