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Turkish Journal of Fisheries and Aquatic Sciences 2014, Vol 14, Num, 3     (Pages: 831-834)

Short-Term Storage of Diluted Fish Sperm in Air Versus Oxygen

Radosław Kajetan Kowalski 1 ,Beata Irena Cejko 1 ,Ilgiz Irnazarow 2 ,Mirosław Szczepkowski 3 ,Stefan Dobosz 4 ,Jan Glogowski 1

1 Polish Academy of Sciences, Institute of Animal Reproduction and Food Research, Department of Gamete and Embryo Biology, Olsztyn, Poland
2 Polish Academy of Sciences, Institute of Ichthyobiology and Aquaculture, Gołysz, Poland
3 Inland Fisheries Institute, Department of Sturgeons Research, Olsztyn, Poland
4 Inland Fisheries Institute, Department of Salmonid Research, Olsztyn, Poland
DOI : 10.4194/1303-2712-v14_3_26 Viewed : 2133 - Downloaded : 2665 Herein we demonstrated the effect of oxygen and air atmosphere on the success of short-term storage of sperm obtained from rainbow trout (Oncorhynchus mykiss), sterlet (Acipenser ruthenus) and common carp (Cyprinus carpio) diluted in immobilizing media (100 mM NaCl, 40 mM KCl, 3 mM CaCl2, 1.5 mM MgCl2, 50mM Tris, pH 8.2 for salmonid fish, 17 mM NaCl, 1.7 mM KCl, 6 mM NaHCO3, 0.5 mM CaCl2, 0.5 mM MgSO4 x 7 H2O, 1.1 mM NaH2PO4 x 2H2O, 1.0 mM Na2HPO4, 50 mM glucose, pH 7.5 for sturgeon, 100 mM NaCl, 3.1 mM KCl, 2 mM CaCl2, 0.4 mM MgCl2, 25 mM NaHCO3, 0.3 mM NaH2PO4; pH 8.64 for cyprinids). Sperm motility of rainbow trout was observed up to 33 days, and percentage of motile spermatozoa was high (70%) up to 14 days after the start of the experiment. In the case of sperm samples which were stored with oxygen, sperm motility (30%) was observed for only two weeks; after this time, sperm motility decreased to 1%. Diluted sturgeon sperm quickly lost their movement potential when stored with oxygen. Samples kept in aerobic conditions maintained 80% of motile spermatozoa after 10 days of short-term storage. In the case of the carp, both, oxygen and aerobic conditions gave similar results within 6 days of the experiment. We assume that short-term storage of diluted fish sperm can be carried out in aerobic conditions which is easier and cheaper in comparison to the pure oxygen atmosphere. Keywords : short-term storage, sperm motility, rainbow trout, carp