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Turkish Journal of Fisheries and Aquatic Sciences 2020, Vol 20, Num, 10     (Pages: 755-766)

Assessment of Some Immune Components from The Bioactive Crude Extract Derived from The Epidermal Mucus of Climbing Perch Anabas Testudines

Agharid al-Rasheed 1 ,Kareem Obayes Handool 2 ,Amaal M Alhelli 3 ,Bashiru Garba 4 ,Belal J Muhialdin 5 ,Malihe Masomian 6 ,Homayoun Hani 7 ,Hassan Hj Mohd Daud 8

1 University of Tikrit, Dept. of food science, Faculty of Agriculture, Iraq
2 Veterinary Directorate, Ministry of Agriculture, Iraq
3 Institute of Technology, Technical of water resources, Iraq
4 Usmanu Danfodiyo University, Faculty of Veterinary Medicine, Dept. of Veterinary Public Health & Preventive Medicine Sokoto, Nigeria
5 Universiti Putra Malaysia, Faculty of Food Science and Technology, UPM Malaysia, Serdang, Selangor Darul Ehsan
6 Universiti Putra Malaysia, Dept. of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Serdang, Selangor Darul Ehsan
7 Universiti Putra Malaysia, Faculty of Veterinary Medicine, Dept. of Veterinary Pathology & Microbiology, Serdang, Selangor Darul Ehsan
8 Universiti Putra Malaysia, Faculty of Veterinary Medicine, Dept. of Clinical Studies, Serdang, Selangor Darul Ehsan
DOI : 10.4194/1303-2712-v20_10_05 Viewed : 179 - Downloaded : 112 Fish and other members of the aquatic habitats are usually in constant interaction with their habitat and potentially harmful pathogens. This study was undertaken to evaluate some of the immune components in the bioactive crude obtained from epidermal mucus of climbing perch. The enzyme activities were assessed using the spectrophotometer and turbidometric assay for the lysozyme activity, and while antimicrobial peptides were determined via AKTA Purifier 10 FPLC with a Superdex™ peptide 10/300 GL column and Q-TOF LC/MS for characterization. Likewise, the antibacterial activity against P. aeruginosa ATCC 10451 was achieved using chromatography fractions. Results showed that higher activities of lysozyme and protease were present in the crude extract. While values for alkaline phosphatase and esterase activities varied. The data analysis of Q-TOF LC/MS showed apolipoprotein A-1, haemoglobin subunit beta (Fragment), elongation factor 1-alpha, cytochrome C oxidase subunit1 (Fragment), Beta-actin (Fragment) and potent antimicrobial peptides including de novo AAGPKGPLGPR peptide in crude. This result emphasizes their role in protecting to climbing perch from foreign invaders as a part of the innate immune system throughout the epidermal mucus. The bioactive crude has a high potential to be used as a natural antimicrobial agent for pharmaceutical applications. Keywords : Mucosal immunity, Antimicrobial peptides, Climbing perch, Aquaculture