Turkish Journal of Fisheries and Aquatic Sciences
2017, Vol 17, Num, 3 (Pages: 565-579)
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Hypoxia-Induced Gene Expression Profiling in The Liver of Freshwater Fish, Channa Striatus
1 ICAR-Central Institute of Freshwater Aquaculture, Fish Genetics and Biotechnology Division, Kausalyaganga, Bhubaneswar 751002, Odisha, India
2 Siksha O Anusandhan University, Centre of Biotechnology, Khandagiri Square, Bhubaneswar, Odisha 751003 India
Channa striatus is a large-bodied fish of freshwater habitat capable of withstanding hypoxic conditions. Adaptive
response to hypoxia is a complex physiological process. A laboratory-based rearing protocol for investigating long-term
hypoxia stress tolerance in this species was established. Using suppression subtractive hybridization technique, we examined
gene expression patterns in liver during prolonged hypoxia exposure. A total of 130 transcripts from the enriched cDNA
library, under hypoxic condition, were sequenced. BLAST analysis identified 58%, 18% and 24% as known, uncharacterized
and unknown sequences, respectively. All known genes represented a broad spectrum of biological pathways such as
transcription/translation, signal transduction, electron transport, immune response, reproduction, cellular transportation.
Heightened abundances for 11 Known, 1 uncharacterized and 1 unknown mRNA in the hypoxic liver were documented.
Among these, the full-length cDNA sequences for heat shock protein 90 and CSHL-338 clone (uncharacterized) were
generated using RACE strategy. Full-length cDNA sequences of prefoldin and fatty acid binding protein was obtained from
the respective clones of SSH cDNA library, were also up-regulated during hypoxia stress. In this study, possible physiological
significances about hypoxia-tolerance transcripts have been discussed. The ESTs presented here will have potential future
implications in exploring new mechanisms of hypoxia acclimation and/or tolerance in C. striatus.
Hypoxia, Channa striatus, SSH, gene expression, gene ontology