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¹ Lishui University, College of Agriculture and Biotechnology, Lishui, Zhejiang 323000, China
² Zhejiang Marine Fisheries Research Institute, Zhoushan, Zhejiang 316100, China DOI : 10.4194/TRJFAS27508 Viewed : 184 - Downloaded : 178 This study evaluated the genetic diversity and population structure of six Pelodiscus sinensis populations in China through mitochondrial DNA 16S rRNA gene sequences and simple sequence repeat (SSR) analyses. The populations include HH (Huanghe population), JP (Japan population), DQ (Deqing population), SY (Songyang population), DT (Dongtinghu population), and HS (Huangsha population). In the 16S rRNA analysis, 49 haplotypes were identified among 138 individuals, with haplotype diversity (Hd) ranging from 0.642 to 0.989 and nucleotide diversity (Pi) from 0.0050 to 0.0110 (Hd>0.5, Pi>=0.005), indicating high genetic diversity. SSR analysis revealed an average observed heterozygosity (Ho) of 0.617 and an expected heterozygosity (He) of 0.800, with a polymorphism information content (PIC) of 0.857, further confirming high genetic diversity. Analysis of molecular variance (AMOVA) showed that 34.75% (16S rRNA gene) and 30.53% (SSR) of the total genetic variation was among populations, while 65.25% (16S rRNA gene) and 69.47% (SSR) was within populations, highlighting that most genetic variation occurs within rather than between populations. Genetic differentiation index (Fst) analysis indicated Fst values mostly between 0.05 and 0.15, suggesting moderate genetic differentiation among most populations. The genetic data provided herein are crucial for the development and utilization of P. sinensis resources. Keywords : TRJFAS27508