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Turkish Journal of Fisheries and Aquatic Sciences
Turkish Journal of Fisheries and Aquatic Sciences 9: 215-221 (2009)
[DOI: 10.4194/trjfas.2009.0214] | | | | Atresia and Apoptosis in Preovulatory Follicles in the Ovary of Danio rerio (Zebrafish) |
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| Sema İşisağ Üçüncü1, Özlem Çakıcı1,* |
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| 1 Ege University, Science Faculty, Biology Department, Zoology Section, 35100 Bornova-Izmir, Turkey. |
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* Corresponding Author: Tel.: +90.2323884000/2420; Fax: +90.2323881036;
E-mail: ozlem.cakici@ege.edu.tr |
Received 24 October 2008
Accepted 09 June 2009 |
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Abstract:
Although apoptosis, the programmed cell death, is the main mechanisms in follicular atresia and especially postovulatory regression in mammals, its role in fish ovary is still controversial. In order to discuss the possible functions of atresia as a context of apoptosis, the preovulatory follicles of the freshwater teleost, Danio rerio (zebrafish) were investigated by light microscopy. The main characteristics of atresia of vitellogenic oocytes of zebrafish were determined as a significant hypertrophy and hyperplasia of the granulosa cells. The theca cells were also became slightly hyperplasic while the zona radiata was degenerated and the basal membrane located between granulosa and theca was also disintegrated. The aggregation of compacted chromatin in a crescent pattern, and cell fragmentation indicates to formation of apoptotic bodies were evaluated as the morphological evidences for apoptosis in granulosa cells of the oocytes of zebrafish. Towards the end of atresia, the vitellus is fully degenerated and absorbed, some apoptotic bodies were also observed between the translucent clusters of theca cells. Apoptotic process was still continued on when an atrium had been formed. |
| Keywords: Atretic follicle, teleost, Danio rerio, apoptosis. |
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| More: Full Text (PDF - 631 KB) |
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