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¹ Çukurova University, Fisheries Faculty, Balcalı, Adana, Turkey DOI : 10.4194/1303-2712-v15_3_09 Viewed : 4209 - Downloaded : 4621 The effect of nitrogen limitation on the cell density, biomass, chlorophyll a, total carotene, protein and lipid content of Phaeodactylum tricornutum (Bohlin), Bacillariophyceae, cultured in photobioreactors outdoor was investigated. Phaeodactylum tricornutum was cultivated in an appropriate medium as the control group, at the same time it was cultured in a medium of which nitrogen was reduced to 50%. At the end of the study, it was determined that 35.04% lipid with 0.980±0.02 gL^-1 biomass and 8.87% protein content, meanwhile, in the control group, 16.93% lipid, 1.036±0.025 gL^-1 biomass and 31.05% protein were determined. As a result, it was observed that while nitrogen limitation increased to the lipid amount, decreased the biomass and chlorophyll a, total carotene and protein of the cell. Keywords : Phaeodactylum tricornutum, lipid, N limitation, tubular photobioreactor, outdoor culture